Coding
Part:BBa_K5181004:Design
Designed by: Eeshani Umesh Abhyankar Group: iGEM24_IISER-TVM (2024-08-13)
SaSSy_Santalum album
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1347
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1459
Illegal SapI.rc site found at 172
Design Notes
The gene sequence is codon optimized for Pseudomonas putida using GenScript's Codon optimization tool.
Source
Santalum album, NCBI(HQ343276.1)
References
(Bohlman et. al., 2016)